芯片上有超过2万种人类全长蛋白质，覆盖81%的人类基因组ORF区，是目前世界上通量最高的蛋白质芯片。将带有生物素标记的小分子与20K芯片共同孵育，再引入带有CY3或CY5荧光的链霉亲和素，通过检测芯片上的荧光信号，即可确定小分子的直接结合靶蛋白。20K芯片不仅能够筛选出与小分子直接结合的靶点蛋白，还能解决传统方法中由于蛋白丰度低而难以捕捉的药-靶结合问题，更因其具备丰富的蛋白种类，单次实验即可产出多项科研成果，具有极高性价比。

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丨芯片蛋白规格

• 芯片标准：1×25×76 mm硅片片基，蛋白点径~60 μm，体积0.5~1 nL；

• 技术重复：每个蛋白点2次技术重复；

• 荧光检测：可使用单一或多色标签；

• 纯化体系：所有蛋白质在非变性条件下，通过真核酵母表达体系表达纯化；

• 生产条件：温度4-8℃，湿度30~40%点样，4℃过夜固定，-80℃长期保存。

丨20K芯片优势

  1. 筛选直接结合的靶点蛋白，假阳性率较低

  2. 无需克隆构建、细胞培养和转染实验

  3. 芯片覆盖多种蛋白类型，结合蛋白范围更广，性价比较高

  4. 采用酵母表达纯化，蛋白丰度高，可检测到微弱蛋白互作现象

丨研究案例

研究案例1：利用一次芯片实验结果发表两篇高分文章

温州医科大学药学院研究团队使用生物素标记雷公藤红素，利用一次20K人类蛋白组芯片实验的结果寻找到其多个直接作用靶点蛋白，发现雷公藤红素能够直接靶向信号转导及转录激活蛋白3 （STAT3）和过氧化还原蛋白2（Prdx2）。确定雷公藤红素的直接靶点蛋白后，通过功能性实验证明了雷公藤红素在治疗胃癌和保护心脏方面发挥一定的作用。

研究案例2：利用多组学+20K芯片确定HDCA的直接靶点

上海中医药大学联合曙光医院及中科院上海药物研究所研究团队通过代谢组学发现代谢物猪脱氧胆酸（HDCA）具有改善NAFLD的潜在作用。随后通过蛋白质组学及转录组学探索其作用机制，发现HDCA的干预显著活化了脂肪酸氧化以及PPARα信号通路。进一步利用20K人类蛋白组芯片筛选HDCA的直接作用靶点，发现HDCA通过结合肝细胞内RAN蛋白，减少RAN/CRM1/PPARα三聚体的形成，抑制PPARα出核，促进脂肪酸氧化，从而治疗NAFLD。

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